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In the case of prelabeled (i.e., with DiI and DiO samples), imaging was performed directly. 3d modeli balyasin. Cultured MSCs were detached using 0.05% Trypsin/EDTA (Invitrogen, Carlsbad, CA, ), resuspended in EBM‐2MV media, added to the EC monolayers, and incubated at 37°C and 5% CO 2 for indicated and then fixed with 3.7% formaldehyde. As indicated in some cases, ECs were preincubated with blocking anti‐VCAM‐1 antibody (20 μg/ml; R&D Systems, Minneapolis, MN, ) for 30 minutes at 37°C and MSCs were treated with PTX (100 ng/ml; Sigma‐Aldrich, St. In some cases, EC and/or MSC were prelabeled with membrane dyes DiI and DiO (1 μg/ml), respectively, for 30 minutes at 37°C, prior to coincubation. Louis, MO, ) for 2 hours at 37°C.
Contents • • • • • • • • • Plot [ ] Chandrakanta is born to Ratnaprabha (), a great Ayyara(magician), who has the most powerful dagger of Lord Vishnu. Iravati (), an evil queen wants the dagger to rule the whole world and conquers. Ratnaprabha stores the dagger at Lord Vishnu's temple to protect it and pass it to Chandrakanta after 21 years.
21 Years Later [ ] 21 years later, Iravati is still searching the dagger while Veerendra / Veer (), her step son awes to her words and plans to conquer Suryagarh kingdom. Chandrakanta (), raised by a poor fisherman couple, gets the dagger, but she doesn't know what she had acquired.
Iravati emotionally blackmails Veer that she is suffering from black magic done by a girl with dagger mark on her body. He goes to Suryagarh and initially, pretends to be a lover of Chandrakanta in order to take revenge but as time went by, developed soft feelings and starts loving her. After a lot of events and turns, Queen Iravati finally succeeded to kill Chandrakanta and gets the Dagger.
Few months later [ ] Chandrakanta returns as Suryakanta () with a revamped avatar to find out that Veer had forgotten about the past time he has spent with her and has become a ruthless warrior under Iravati. After a long hurdle Veer regains his memory and finds out the truth about his mother and Chandrakanta and then decides to oppose his mother. He fights off and defeats the demons sent by his mother and saves Chandrakanta on several occasions and also fights his stepbrother Swayam (), the beast and defeats him in quite a few occasions but is unable to kill him. Finally Veer finds out that Swayam is immortal as blessed by Lord Vishnu and then he gives up his life fighting the beast and protecting Chandrakanta. Chandrakanta then as advised by Lord Vishnu injects Swayam's blood in Veer's body and resurrects him which shows some changes in his eyes which indicates that there is some change which has happened to Veer and in the mean time Tej (), Veer's friend, Umang (), Chandrakanta's friend and Bhadra Maa () gets captured and tortured by Iravati to find out Chandrakanta.
After resurrecting Veer back to life Chandrakanta finds out that Veer has received a new form of power which is the form of Narsimha a powerful form like the Avatar of Lord Vishnu and finds out that Veer is the divine warrior and is chosen to put an end to the despotic rule of Queen Iravati and Swayam. Finally Chandrakanta teaches Veer to learn to control his new found powers and finally after a long fight Veer takes the form of the great Narsimha and kills Swayam and finally puts an end to the terror of Hirnasur. However Iravati escapes and sorts the help of the 'Asurs' from the Paatal Lok to defeat Veer and in the mean time Veer and Chandrakanta establishes peace and prosperity in Vijaygarh.